Behavior of a cross-linked attachment site: Testing the role of branch migration in site-specific recombination

  • Cowart M
  • Benkovic S
  • Nash H
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Abstract

Integrative recombination of bacteriophage lambda requires perfect homology between partners over a short segment of DNA, the overlap region, that separates the positions of top and bottom strand exchange. We constructed a specific cross-link between complementary strands in the overlap region of one partner, using a method designed to introduce minimal distortion of DNA. The modified attachment site could initiate recombination, forming a Holliday junction, but could not resolve this junction so as to complete the recombination. This demonstrates that the ability of complementary base-pairs to dissociate is important for overlap region function and strongly supports the view that branch migration across this region is the way homology is sensed during integrative recombination. © 1991.

Author-supplied keywords

  • Holliday junction
  • bacteriophage lambda
  • branch migration
  • cross-linking
  • site-specific recombination

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Authors

  • Marlon Cowart

  • Stephen J. Benkovic

  • Howard A. Nash

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