BHV-1 glycoprotein 1 and recombinant interleukin 1β efficiently elicit mucosal IgA response

  • Gao Y
  • Daley M
  • Splitter G
  • 4

    Readers

    Mendeley users who have this article in their library.
  • 18

    Citations

    Citations of this article.

Abstract

The mucosal immune response to most soluble antigens administered directly to the mucosal system is low and requires a large amount of antigen and frequent vaccinations. In this study we tested whether immunizing cattle at a site which shares lymphatic drainage with the nasal mucosa could prime local mucosal immunity. We further tested whether recombinant bovine IL-1β (rBoIL-1β) could potentiate the induction of mucosal immunity. Animals were immunized subcutaneously at the base of the ear (s.e.) with recombinant bovine herpesvirus-1 (BHV-1) envelope glycoprotein I (gI) (35 μg animal-1) emulsified in incomplete Freund's adjuvant with or without rBoIL-1β (500 ng kg-1) followed by a second immunization 42 days later. Animals were challenged with virulent BHV-1 intranasally 42 days after the second immunization. Mucosal IgA from the nares was induced after only one immunization and, enhanced by boosting. rBoIL-1β treated animals had higher levels of BHV-1 specific nasal IgA (p

Author-supplied keywords

  • B lymphocytes
  • Bovine herpes virus-1
  • IgA
  • antibody
  • interleukin 1β
  • mucosal immunity

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Yi Gao

  • Michael J. Daley

  • Gary A. Splitter

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free