The binding of calcium to human fibronectin.

  • Amphlett G
  • Hrinda M
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Abstract

The binding of calcium to human plasma fibronectin has been measured by equilibrium dialysis at 25 degrees in 0.1 M NaCl 50mM Tris HCL, pH 7.4. Curve fitting of the binding data indicates that fibronectin has two strong calcium binding sites per chain (Mr 220,000), KD = 1.3 mM and approximately 12 weak sites, KD = 2.3 mM. No significant displacement of bound calcium by magnesium was observed at magnesium concentrations up to 1 mM. Calcium binding to a pair of tryptic fragments of fibronectin (Mr congruent to 160,000 and 180,000) that bind to gelatin has also been investigated. These fragments have a single class of calcium binding sites, with 2.2 sites per chain, KD = 1.1 mM. Negligible calcium binding to tryptic fragments derived from other regions of the fibronectin molecule was observed.

Author-supplied keywords

  • Binding Sites
  • Calcium
  • Calcium: blood
  • Fibronectins
  • Fibronectins: blood
  • Humans
  • Light
  • Protein Binding
  • Radiation
  • Scattering

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Authors

  • G W Amphlett

  • M E Hrinda

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