Bryostatins trigger human polymorphonuclear neutrophil and monocyte oxidative metabolism: association with in vitro antineoplastic activity

  • Esa A
  • Warren J
  • Hess A
 et al. 
  • 3

    Readers

    Mendeley users who have this article in their library.
  • 4

    Citations

    Citations of this article.

Abstract

Bryostatin-1 - but not bryostatin-13 - a macrocyclic lactone isolated from the marine bryozoan Bugula neritina, triggered human polymorphonuclear neutrophil (PMN) and monocyte release of reactive oxygen radicals, as measured by the generation of lucigenin chemiluminescence and by the ferricytochrome c reduction assay. The release of oxygen radicals by bryostatins was sensitive to inhibitors of protein kinases, but resistant to the inhibition of phospholipase A2activity and arachidonic acid metabolism (prior treatment with mepacrine or indomethacin). Comparison of the effect of protein kinase (PK) inhibitors H-8, H-7 and staurosporine on bryostatin-1-induced neutrophil oxygen radical release further suggested a requirement for activation of phospholipid-dependent PKC, but not for cGMP- or cAMP-dependent PK. In cytostatic assays, PMNs treated with bryostatin-1 inhibited the growth of the erythroleukaemic cell line K562 in a concentration-dependent manner. These findings suggest that the reported antineoplastic effect of bryostatins may result, at least in part, from activation of PMNs and monocytes. © 1995 Institut Pasteur/Elsevier, Paris.

Author-supplied keywords

  • Bryostatin, Oxygen, Cytostasis, Polymorphonuclear leukocyte
  • Reactive oxygen radicals, Tumoricidal effect, Protein Kinase, Monocytes

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • A. H. Esa

  • J. T. Warren

  • A. D. Hess

  • W. S. May

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free