Liquid chromatographic separation in capillary tubing of particles ranging from 0.5 to 30 μm is reported. Elution volumes are inversely related to particle diameter for materials of diverse composition such as latex, pollen, bacterial spores, silica, and whole cells. Relative elution times of particles are dependent on column diameter and both the velocity and viscosity of the mobile phase. Viscosity appears to affect the relative retention of small particles more than particles over 1 μm in diameter. Addition of ethyleen glycol to aqueous mobile phases diminishes peak trailing wiht latex particles. The practical limit on column length is approximately 300 ft. © 1978.
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