Cauliflower mosaic virus 35 S RNA leader region inhibits translation of downstream genes

25Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The cauliflower mosaic virus (CaMV) 35 S RNA is a full-length transcript of the viral genome. It encodes the genes VII and I-V, arranged in tandem along the RNA, preceded by a long leader region (600 bases) containing many short open reading frames. We have examined the effects of the leader and the first gene (gene VII) on downstream gene I translation in vitro and in an in vivo transient expression system (carrot protoplasts). RNAs from constructs containing the intact leader, and from various deletion constructs, were translated in a rabbit reticulocyte system. Gene I was translated efficiently only when the long leader region and the upstream gene VII were deleted. Translational fusions of gene VII or I to the firefly luciferase reporter gene were also constructed, and a similar series of leader sequence deletion mutants were examined in vivo and in vitro. The 600-base leader region was found to repress translation of gene VII 8- to 30-fold as compared to the truncated gene lacking the leader region. Gene I expression as compared to that of gene VII was reduced an additional 7- to 20-fold by the presence of the upstream leader region including gene VII. This represented an overall reduction in gene I expression of greater than 100-fold as compared to expression in the absence of any leader sequence. The reduced translation of gene I in the context of the 35 S RNA leader region was not due to the action of the gene VII protein product but may result from efficient blocking of scanning 40 S ribosomes by translation of upstream open reading frames. © 1988.

Cite

CITATION STYLE

APA

Baughman, G., & Howell, S. H. (1988). Cauliflower mosaic virus 35 S RNA leader region inhibits translation of downstream genes. Virology, 167(1), 125–135. https://doi.org/10.1016/0042-6822(88)90061-X

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free