Abscisic acid (ABA) inhibited the light-induced opening of stomata in isolated epidermal strips of Commelina benghalensis. It did not alter stomatal closure in the dark. The ABA-induced inhibition in light was released under conditions conducive for cyclic photophosphorylation and remarkably reversed by ATP in the presence of pyruvate. Cyclic photophosphorylation rates of isolated guard cell chloroplasts were significantly reduced by ABA. It is proposed that the direct effect of ABA on stomatal opening was mediated in two ways: (1) by inhibition of cyclic photophosphorylation activities of guard cell chloroplasts and (2) by blocking organic acid formation in guard cells. © 1976.
Raghavendra, A. S., Rao, I. M., & Das, V. S. R. (1976). Characterisation of abscisic acid inhibition of stomatal opening in isolated epidermal strips. Plant Science Letters, 6(2), 111–115. https://doi.org/10.1016/0304-4211(76)90144-9