Characterization of maintained voltage-dependent K+-channels induced in Xenopus oocytes by rat brain mRNA

  • Hoger J
  • Rudy B
  • Lester H
 et al. 
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Abstract

The voltage-dependent K+currents encoded by rat brain mRNA were studied in Xenopus oocytes after the voltage-dependent Na+currents and the Ca2+-activated Cl-currents were eliminated pharmacologically. This paper describes the maintained K+currents (IK), defined primarily by resistance to inactivation for 1 s at a holding potential of -40 mV. IKactivates at potentials more positive than -60 to -70 mV and consists of both low-threshold and high-threshold components. IKis partially blocked by both tetraethyl ammonium (TEA) and 4-aminopyridine (4-AP), which appear to be blocking the same component. Long depolarizing pulses result in incomplete inactivation of IK; the inactivating component is inhibited by TEA. Sucrose density gradient fractionation partially resolves the RNA encoding the several components of IK; most IKarises from size classes between 3.8 and 9.5 kb. The study gives further evidence for the existence of numerous distinct RNA populations that encode brain K+channels different from previously reported cloned K+channels that have been expressed in Xenopus oocytes. © 1991.

Author-supplied keywords

  • Ion channel
  • Potassium channel
  • RNA fractionation
  • Xenopus oocyte
  • delayed rectifier
  • mRNA

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Authors

  • Jeff H. Hoger

  • Bernardo Rudy

  • Henry A. Lester

  • Norman Davidson

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