Characterization of the Pseudomonas aeruginosa transposable bacteriophage D3112 A and B genes

  • Ulycznyj P
  • Salmon K
  • Douillard H
 et al. 
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Abstract

The left end DNA of Mu-like transposable bacteriophage 133112 was sequenced from by 2521 to by 5483. Two large open reading frames were identified: ORF A (bp 2539-4611) and ORF B (bp 4626-5378). ORF A can encode a 690 amino acid, 78 kDa protein which is 44.4% similar to Mu transposase and ORF B can encode a 250 amino acid, 27 kDa protein, which is 46.4% similar to, though 62 amino acids shorter than, the Mu B protein. The cloned 133112 A gene exhibited activity on a mini-D3112-containing plasmid in Pseudomonas aeruginosa. © 1995.

Author-supplied keywords

  • A gene
  • B gene
  • Bacteriophage 133112
  • Transposase

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Authors

  • Peter I. Ulycznyj

  • Kirsty A. Salmon

  • Hélène Douillard

  • Michael S. DuBow

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