The chemo-enzymatic synthesis and evaluation of oligosaccharide-branched cyclodextrins

  • Matsuda K
  • Inazu T
  • Haneda K
 et al. 
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The syntheses of oligosaccharide-branched cyclodextrins, which showed potential binding to the saccharide-interacting protein, lectin, were investigated. The transglycosylations to Fmoc-Asn(GlcNAc)-NH-cyclodextrin by endo-β-N-acetylglucosaminidase of Mucor hiemalis (Endo-M) gave three kinds of natural oligosaccharide-branched cyclodextrins (7-9) in satisfactory yields. The association constant of the high-mannose type oligosaccharide-branched CD (7) with immobilized concanavalin A was found to be approximately 1.6 x 107M-1using an optical biosensor.

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  • Toshiyuki INAZUTokai University - Shonan Campus

  • Keisuke Matsuda

  • Katsuji Haneda

  • Mamoru Mizuno

  • Takashi Yamanoi

  • Kenjiro Hattori

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