The syntheses of oligosaccharide-branched cyclodextrins, which showed potential binding to the saccharide-interacting protein, lectin, were investigated. The transglycosylations to Fmoc-Asn(GlcNAc)-NH-cyclodextrin by endo-β-N-acetylglucosaminidase of Mucor hiemalis (Endo-M) gave three kinds of natural oligosaccharide-branched cyclodextrins (7-9) in satisfactory yields. The association constant of the high-mannose type oligosaccharide-branched CD (7) with immobilized concanavalin A was found to be approximately 1.6 x 107M-1using an optical biosensor.
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