Polyethyleneimine has been attached to epoxide-activated Sepharose. The resulting material exhibits high capacity, fast equilibration on acid-base titration and no maxima in a pH gradient. The resolving power is improved when some of the imino groups are converted into guanidino groups on modification with O-methylisourea. The final material is suitable for separations at pH ≦9.2. © 1981.
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