Chromatofocusing. IV. Properties of an agarose polyethyleneimine ion exchanger and its suitability for protein separations

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Abstract

Polyethyleneimine has been attached to epoxide-activated Sepharose. The resulting material exhibits high capacity, fast equilibration on acid-base titration and no maxima in a pH gradient. The resolving power is improved when some of the imino groups are converted into guanidino groups on modification with O-methylisourea. The final material is suitable for separations at pH ≦9.2. © 1981.

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Sluyterman, L. A. Æ., & Wijdenes, J. (1981). Chromatofocusing. IV. Properties of an agarose polyethyleneimine ion exchanger and its suitability for protein separations. Journal of Chromatography A, 206(3), 441–447. https://doi.org/10.1016/S0021-9673(00)88913-X

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