A heatstable alpha amylase gene was shotgun cloned from Bacillus licheniformis RP01 into Bacillus subtilis. Restriction endonuclease analysis of the recombinant plasmid revealed a map which was identical to a previously cloned alpha amylase from B. licheniformis FD02 and very similar to the restriction map of a high temperature amylase from Bacillus coagulans. The thermostability and temperature optimum of the cloned alpha amylase was measureably different from those of the previously reported cloned alpha amylases. © 1984.
Piggott, R. P., Rossiter, A., Ortlepp, S. A., Pembroke, J. T., & Ollington, J. F. (1984). Cloning in Bacillus subtilis of an extremely thermostable alpha amylase: Comparison with other cloned heatstable alpha amylases. Biochemical and Biophysical Research Communications, 122(1), 175–183. https://doi.org/10.1016/0006-291X(84)90456-X