A freshly isolated strain of Plasmodium falciparum was cloned by limited dilution using a co-culture of infected erythrocytes on monolayers of functionally active rodent hepatocytes. 15 clones were isolated, and the anti-malarial activity of chloroquine, quinine, mefloquine and halofantrine against the clones, the original isolate, and a culture-adapted isolate was determined using a 48 h radioisotope microdilution method. The multiplication rates of all clones and the culture-adapted isolate were estimated by counting the number of parasitized cells on Giemsa-stained thin smears. Variations found in drug sensitivity and multiplication rate of different clones provided strong evidence of heterogeneity of a single strain parasite population. No morphological variation was detected by light microscopy. The use of a hepatocyte feeder layer improved the adaptation of cloned parasites to continuous culture conditions and thus enabled us to clone directly a fresh isolate, without losing clones during the culture-adaptation process. © 1990, Royal Society of Tropical Medicine and Hygiene. All rights reserved.
CITATION STYLE
Mirovsky, P., Gay, F., Bustos, D., Mazier, D., & Gentilini, M. (1990). Cloning of a fresh isolate of Plasmodium falciparum and drug sensitivity of the clones. Transactions of the Royal Society of Tropical Medicine and Hygiene, 84(4), 511–515. https://doi.org/10.1016/0035-9203(90)90018-A
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