Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver

  • Pirrit L
  • Healey R
  • Assheton H
 et al. 
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Abstract

cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyltransferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol UDPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than those of mammals, is repressed after 3-MC treatment. © 1995.

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Authors

  • L. Pirrit

  • R. Healey

  • H. Assheton

  • M. J. Leaver

  • S. G. George

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