Cloning and sequencing of a human cDNA from cytolytic NK/T cells with homology to yeast SEC7

  • Liu L
  • Pohajdak B
  • 5

    Readers

    Mendeley users who have this article in their library.
  • 64

    Citations

    Citations of this article.

Abstract

Using the technique of subtractive hybridization (natural killer (NK) minus T helper cell line Jurkat), a human cDNA clone named B2-1 was isolated and sequenced. B2-1 transcripts are highly expressed in NK and peripheral T cells, but not in the T helper cell line used in the subtraction. Two overlapping cDNA clones contained 3309 bp, in agreement with a predicted size of 3.2 kb obtained by Northern blot analysis. The cDNA contained a 5′ open reading frame encoding a 398 amino acid (aa) protein with a calculated Mrof 46 383. The protein contains N-linked glycoylation sites and a site for phosphorylation by protein kinase C. Database homology searches show that the deduced protein is novel and has three discrete domains with significant homology to several structural proteins. The most striking homology was to yeast SEC7 in the central domain of the gene (57% identical over 466 bp) and also the protein level (42% identical amino acids; 39% conserved amino acids). SEC7 is a large protein (2008 amino acids) found on Golgi vesicles that plays a role in protein transport. © 1992.

Author-supplied keywords

  • Golgi apparatus
  • Natural killer cell
  • Subtractive hybridization
  • T cell
  • cDNA cloning

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Lin Liu

  • Bill Pohajdak

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free