An aqueous high-performance size-exclusion chromatographic (HPASEC) method for lignosulphonates was developed and optimized using a TSK G3000SW column. Curie-point pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) was used to characterize the lignosulphonates on the molecular level. Combined HPASEC and Py-GC-MS results showed that a sodium lignosulphonate standard, prepared under mild conditions, was a relatively polydisperse polymer that still contained features of a natural lignin polymer. Lignosulphonates discharged by pulp mills are more monodisperse macromoles of a relatively low molecular mass and are structurally modified to a greater extent than the sodium lignosulphonate standard. A relationship was established between the molecular mass distributions of the various lignosulphonate macromolecules and the fraction of preserved phenylpropane structural units. © 1993.
van der Hage, E. R. E., van Loon, W. M. G. M., Boon, J. J., Lingeman, H., & Brinkman, U. A. T. (1993). Combined high-performance aqueous size-exclusion chromatographic and pyrolysis-gas chromatographic-mass spectrometric study of lignosulphonates in pulp mill effluents. Journal of Chromatography A, 634(2), 263–271. https://doi.org/10.1016/0021-9673(93)83013-I