Comparison of glutamate and N-methyl-D-aspartate toxicities on rat mesencephalic primary cell cultures

  • Mateu G
  • Privat A
  • Thibault J
 et al. 
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Abstract

Excitotoxicities of glutamate and NMDA were studied on primary cultures of rat embryonic substantia nigra. The toxicity of the general neuronal population (identified with neuron specific enolase - NSE) was compared with that of dopaminergic neurons (identified with TH antibodies). We have shown that there exists a time-dependent toxicity to glutamate in 9 d old cultures in vitro and exposures as short as 5 min are significantly toxic. By comparing the effects of long time exposures (24 h) to NMDA and glutamate, we can show dose-dependent toxicity; however NMDA shows a less marked effect, especially at high doses (> 500-1000 μM) as opposed to less potent lower doses (< 500 μM). In comparison to the general population of NSE-positive mesencephalic neurons, TH-positive neurons seem to exhibit a similar vulnerability to EAA. The fact that TH-positive neurons are only partially protected against glutamate toxicity by the non-competitive NMDA antagonist TCP indicates that they are more susceptible to non-NMDA mediated neurotoxicity than the general neuronal population.

Author-supplied keywords

  • Dopaminergic neurons
  • Excitotoxicity
  • Glutamate
  • N-methyl-D-aspartate
  • Substantia nigra cell culture
  • TCP

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Authors

  • Guylaine Mateu

  • Alain Privat

  • Jean Thibault

  • Jacques Vignon

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