Comparison of glutamate and N-methyl-D-aspartate toxicities on rat mesencephalic primary cell cultures

  • Mateu G
  • Privat A
  • Thibault J
 et al. 
  • 2


    Mendeley users who have this article in their library.
  • 4


    Citations of this article.


Excitotoxicities of glutamate and NMDA were studied on primary cultures of rat embryonic substantia nigra. The toxicity of the general neuronal population (identified with neuron specific enolase - NSE) was compared with that of dopaminergic neurons (identified with TH antibodies). We have shown that there exists a time-dependent toxicity to glutamate in 9 d old cultures in vitro and exposures as short as 5 min are significantly toxic. By comparing the effects of long time exposures (24 h) to NMDA and glutamate, we can show dose-dependent toxicity; however NMDA shows a less marked effect, especially at high doses (> 500-1000 μM) as opposed to less potent lower doses (< 500 μM). In comparison to the general population of NSE-positive mesencephalic neurons, TH-positive neurons seem to exhibit a similar vulnerability to EAA. The fact that TH-positive neurons are only partially protected against glutamate toxicity by the non-competitive NMDA antagonist TCP indicates that they are more susceptible to non-NMDA mediated neurotoxicity than the general neuronal population.

Author-supplied keywords

  • Dopaminergic neurons
  • Excitotoxicity
  • Glutamate
  • N-methyl-D-aspartate
  • Substantia nigra cell culture
  • TCP

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Guylaine Mateu

  • Alain Privat

  • Jean Thibault

  • Jacques Vignon

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free