Comparison of glutamate and N-methyl-D-aspartate toxicities on rat mesencephalic primary cell cultures

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Abstract

Excitotoxicities of glutamate and NMDA were studied on primary cultures of rat embryonic substantia nigra. The toxicity of the general neuronal population (identified with neuron specific enolase - NSE) was compared with that of dopaminergic neurons (identified with TH antibodies). We have shown that there exists a time-dependent toxicity to glutamate in 9 d old cultures in vitro and exposures as short as 5 min are significantly toxic. By comparing the effects of long time exposures (24 h) to NMDA and glutamate, we can show dose-dependent toxicity; however NMDA shows a less marked effect, especially at high doses (> 500-1000 μM) as opposed to less potent lower doses (< 500 μM). In comparison to the general population of NSE-positive mesencephalic neurons, TH-positive neurons seem to exhibit a similar vulnerability to EAA. The fact that TH-positive neurons are only partially protected against glutamate toxicity by the non-competitive NMDA antagonist TCP indicates that they are more susceptible to non-NMDA mediated neurotoxicity than the general neuronal population.

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Mateu, G., Privat, A., Thibault, J., & Vignon, J. (1997). Comparison of glutamate and N-methyl-D-aspartate toxicities on rat mesencephalic primary cell cultures. International Journal of Developmental Neuroscience, 15(7), 851–865. https://doi.org/10.1016/S0736-5748(97)00038-5

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