A competitive avidin-biotin ECL dot-blot assay for epitope mapping of monoclonal antibodies

  • Tarassishin L
  • Russell W
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Abstract

Using antigen-soaked nitrocellulose sheets stamped to Teraski plates, a microtechnique was devised for detecting bound biotinylated monoclonal antibodies which readily demonstrated non-overlapping epitopes. This procedure is simple, sensitive and rapid and is useful in screening hybridoma supernatants. © 1994.

Author-supplied keywords

  • Biotin
  • ECL
  • Epitope mapping
  • Monoclonal antibody

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Authors

  • L. A. Tarassishin

  • W. C. Russell

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