Copy number and chromosomal location of Saccharomyces cerevisiae tRNATrp-encoding genes

  • Yesland K
  • Daemyung K
  • Johnson J
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Abstract

Yeast tRNATrp-encoding genes have been identified by Southern-blot analysis of chromosomal DNA. Seven copies of this gene are detected in blots of both restriction endonuclease digests and intact chromosomal DNA. Electrophoretic mapping of tDNATrpindicates one copy is located on each of chromosomes X, XI, XIII, and XVI. The remaining three copies are localized to chromosomes VII and/or XV. Three different yeast strains gave identical results indicating this multi-gene family is relatively stable. © 1991.

Author-supplied keywords

  • Recombinant DNA
  • gene mapping
  • multicopy gene
  • pulsed-field electrophoresis
  • yeast

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Authors

  • Kyle Yesland

  • Kim Daemyung

  • Jerry D. Johnson

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