In providing chemiluminescent probes that have high chemiluminescence intensity and high specificity to superoxide anions, novel chemiluminescent probes involving cyclodextrins covalently bound to 6-(4-methoxyphenyl)imidazo[1, 2-α]pyrazin-3(7H)-one with fluorescein were synthesized and characterized. Using the hypoxanthine-xanthine oxidase system for the generation of the superoxide anions, these novel chemiluminescent probes showed higher superoxide-induced chemiluminescence intensity than that of 6-[4-[2-[N′- (5-fluoresceinyl)thioureido]-ethoxy]phenyl]-2-methylimidazo[1,2-α] pyrazin-3(7H)-one (FCLA). When tested at a probe concentration of 1.0μM, compound 6, in which 6-(4-methoxyphenyl)imidazo[1,2-α]pyrazin-3(7H)-one and fluorescein are covalently attached on the secondary and primary hydroxyl faces of γ-cyclodextrin, respectively, showed green luminescence intensity that was 26 times that of FCLA, which was also the highest luminescence intensity in this present study. At probe concentrations of less than 1.0μM, the ratio of the superoxide-dependent chemiluminescence intensity to the background chemiluminescence intensity for compound 6 was higher than that of FCLA. This high superoxide-induced chemiluminescence intensity and superoxide specificity in low probe concentrations indicates that 6 can be more effective than FCLA toward the measurement of superoxide anions. © 2003 Elsevier Inc. All rights reserved.
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