Demonstration of highly specific and sensitive antibodies to a naturally occurring tetrahydroisoquinoline alkaloid, salsolidine

  • Stammel W
  • Müller R
  • Thomas H
  • 3

    Readers

    Mendeley users who have this article in their library.
  • 3

    Citations

    Citations of this article.

Abstract

1. 1. We report for the first time on the production and characterization of antibodies against a naturally occurring tetrahydroisoquinoline, namely salsolidine (6,7-dimethoxy-1-methyl-1,2,3,4-tetrahydroisoquinoline). 2. 2. Immunogen synthesis was carried out by coupling the hapten salsolidine to bovine serum albumin (BSA) as carrier protein on the basis of reductive amination. 3. 3. By immunization of rabbits with salsolidine-BSA conjugate antisalsolidine antibodies were produced. 4. 4. At a final dilution of 1:1700 the highest-litre antiserum bound 35% of 0.21 pmol [3H] salsolidine. This antiserum was used to develop a radioimmunoassay for salsolidine. 5. 5. Cross-reactivity studies revealed a high specificity of the antiserum to the hapten. 6. 6. The antibodies had a high affinity to salsolidine (Ka= 1.5 × 109M-1). 7. 7. Standard curves covered a measuring range of 0.5-70 pmol/tube and the detection limit was found to be 0.27 pmol/tube. © 1993.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Waltraud Stammel

  • Robert Müller

  • Helmut Thomas

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free