Depth-dependent analysis of membranes using benzophenone-based phospholipids

  • John B
  • Kumar E
  • Lala A
  • 5

    Readers

    Mendeley users who have this article in their library.
  • 3

    Citations

    Citations of this article.

Abstract

Any attempt to probe the membrane hydrophobic core with chemical reagents necessitates the use of reactive intermediates like carbenes and nitrenes, which can insert into C-H bonds. Several photoactivable reagents based on carbenes and nitrenes have been reported. However, the high reactivity of these reagents, often leads to very low insertion yields. We report here a high degree of cross-linking (35-40%) achieved with three benzophenone-based phospholipids and analyze the carbon functionalization data using a multiple Gaussian function. These phospholipids are so designed so as to permit depth-dependent labeling in membranes. Single bilayer vesicles were prepared from these phospholipids and dimyristoylphosphatidylcholine. The cross-linked product was isolated and characterized by mass spectroscopy. The results obtained indicated that the cross-linked product was dominated by dimeric product formed by intermolecular cross-linking. The Gaussian analysis used here provides insight into the relative depths of the probes inside the membrane. (C) 2000 Elsevier Science B.V.

Author-supplied keywords

  • Benzophenone-based phospholipids
  • Cross-linking
  • Depth-dependent analysis
  • Gaussian

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Bino John

  • E. Ravi Kumar

  • Anil K. Lala

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free