Detection of foot-and-mouth disease virus by competitive ELISA using a monoclonal antibody specific for the 12S protein subunit from six of the seven serotypes

  • Smitsaart E
  • Saiz J
  • Yedloutschnig R
 et al. 
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Abstract

Foot-and-mouth disease (FMD) prevention and control programs are dependent upon rapid, reliable diagnostic procedures. The widely used FMD diagnostic complement fixation (CF) procedures require a specific antiserum for each of the seven FMDV serotypes making the tests both cumbersome and difficult to standardize. An FMD diagnostic, monoclonal antibody based inhibition-ELISA procedure was developed. The test uses a single monoclonal antibody (MAb) that reacts with all European and South American FMDV isolates examined. The procedure detects a highly conserved epitope on the 12S protein subunit of FMDV which appears to be common to all FMDV's with the exception of the South African Territories 2 serotype. The results indicate that the sensitivity of this test is greater than CF and approaches that of virus isolation.

Author-supplied keywords

  • Animals
  • Antibodies, Monoclonal/immunology
  • Antibodies, Viral/*immunology
  • Antibody Specificity/immunology
  • Antigens, Viral/immunology
  • Aphthovirus/classification/*isolation & purificati
  • Cattle
  • Cattle Diseases/diagnosis/microbiology
  • Complement Fixation Tests/veterinary
  • Enzyme-Linked Immunosorbent Assay/methods/*veterin
  • Epitopes/immunology
  • Foot-and-Mouth Disease/diagnosis
  • Mice
  • Mice, Inbred BALB C
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serotyping/veterinary
  • Swine
  • Swine Diseases/diagnosis/microbiology
  • Viral Proteins/*immunology

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Authors

  • E N Smitsaart

  • J C Saiz

  • R J Yedloutschnig

  • D O Morgan

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