Sameridine is a new candidate drug with both local anaesthetic and analgesic properties. The free concentration of sameridine in blood plasma was determined by coupled-column liquid chromatography. Following adjustment of the pH and the temperature of the plasma samples, the free fraction was prepared by ultrafiltration. The coupled-column liquid chromatographic system consisted of a reversed-phase column, a cation-exchange extraction column and a cation-exchange analytical column. Sameridine was detected by UV determination at 205 nm and the system showed high selectivity. The limit of quantification was 1 nM and the within-day precision was 4.6% (R.S.D., n= 10).
Eklund, E., Norsten-Höög, C., & Arvidsson, T. (1998). Determination of free concentration of sameridine in blood plasma by ultrafiltration and coupled-column liquid chromatography. Journal of Chromatography B: Biomedical Applications, 708(1–2), 195–200. https://doi.org/10.1016/S0378-4347(97)00671-3