Determination of furosine in biomedical samples employing an improved hydrolysis and high-performance liquid chromatographic technique

  • Cefalu W
  • Bell-Farrow A
  • Wang Z
 et al. 
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Abstract

Traditionally, the most sensitive and specific determination of non-enzymatic protein glycation has involved an 18-24-h acid hydrolysis in order to generate the compound furosine, which has been detected employing reversed-phase h.p.l.c. In this study, we have reported that significant quantities of furosine can be generated with much shorter hydrolysis times employing a 90-min vapor-phase acid hydrolysis procedure. The furosine generated by vapor-phase hydrolysis is then quantitated by pulsed amperometric detection using anion-exchange high-performance liquid chromatography. Employing this method, we were able to show that furosine generated from acid hydrolysis of purified hepatic membranes in a diabetic and non-diabetic animal model agreed with traditional methods assessing total glycated protein (i.e., boronate affinity methods). © 1991.

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Authors

  • William T. Cefalu

  • Audrey Bell-Farrow

  • Zhong Q. Wang

  • Suresh Ralapati

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