Polycyclic aromatic hydrocarbons (PAH) were determined in extracts of zebra mussels sampled from sites in Hamilton Harbour. The mussels were extracted using a tissue homogenizer method and the resulting solvent extracts were subjected to an alumina column and a Sephadex LH20 column clean-up procedure. Total PAH concentrations in Zebra mussel extracts ranged from 0.35 μg/g to 10.0 μg/g of mussel homogenate. The extracts were also found to exhibit significant mutagenic responses in the Ames Salmonella/microtome assay. The results indicate that zebra mussels might be used as biological indicators of PAH contamination in freshwater environments such as Hamilton Harbour. © 1994, International Association for Great Lakes Research. All rights reserved.
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