Determination of hemoglobin through its peroxidase activity on chlorpromazine

  • Vázquez A
  • Tudela J
  • Varón R
 et al. 
  • 5


    Mendeley users who have this article in their library.
  • 14


    Citations of this article.


Chlorpromazine is an excellent chromogen for determining micro-quantities of hemoglobin. The oxidation of chlorpromazine by peroxidase activity of hemoglobin is coupled to a non-enzymatic reaction of second-order. Kinetic analysis of the overall system leads to a discussion about the optimal assay conditions. Spectrophotometric progress curves for the accumulation of the chlorpromazine cation radical during the reaction have been obtained, and further analyzed by non-linear regression. The use of a linear calibration curve of the enzymatic reaction rate against hemoglobin concentration is proposed for its determination. © 1991.

Author-supplied keywords

  • Chlorpromazine
  • Enzyme kinetics
  • Hemoglobin
  • Peroxidase activity
  • Phenothiazine

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • A. Vázquez

  • J. Tudela

  • R. Varón

  • F. García-Cánovas

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free