Determination of hemoglobin through its peroxidase activity on chlorpromazine

14Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Chlorpromazine is an excellent chromogen for determining micro-quantities of hemoglobin. The oxidation of chlorpromazine by peroxidase activity of hemoglobin is coupled to a non-enzymatic reaction of second-order. Kinetic analysis of the overall system leads to a discussion about the optimal assay conditions. Spectrophotometric progress curves for the accumulation of the chlorpromazine cation radical during the reaction have been obtained, and further analyzed by non-linear regression. The use of a linear calibration curve of the enzymatic reaction rate against hemoglobin concentration is proposed for its determination. © 1991.

Cite

CITATION STYLE

APA

Vázquez, A., Tudela, J., Varón, R., & García-Cánovas, F. (1991). Determination of hemoglobin through its peroxidase activity on chlorpromazine. Journal of Biochemical and Biophysical Methods, 23(1), 45–52. https://doi.org/10.1016/0165-022X(91)90049-3

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free