Determination of hemoglobin through its peroxidase activity on chlorpromazine

  • Vázquez A
  • Tudela J
  • Varón R
 et al. 
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Abstract

Chlorpromazine is an excellent chromogen for determining micro-quantities of hemoglobin. The oxidation of chlorpromazine by peroxidase activity of hemoglobin is coupled to a non-enzymatic reaction of second-order. Kinetic analysis of the overall system leads to a discussion about the optimal assay conditions. Spectrophotometric progress curves for the accumulation of the chlorpromazine cation radical during the reaction have been obtained, and further analyzed by non-linear regression. The use of a linear calibration curve of the enzymatic reaction rate against hemoglobin concentration is proposed for its determination. © 1991.

Author-supplied keywords

  • Chlorpromazine
  • Enzyme kinetics
  • Hemoglobin
  • Peroxidase activity
  • Phenothiazine

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Authors

  • A. Vázquez

  • J. Tudela

  • R. Varón

  • F. García-Cánovas

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