Determination of impurities in nucleoside 3′-phosphoramidites by fast atom bombardment mass spectrometry

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Abstract

Negative-ion fast atom bombardment mass spectrometry is quite useful for the identification of products and by-products formed during the synthesis of nucleoside 3′-phosphoramidites. The data shown that detritylation and oxidation are side reactions which occur during the synthesis of monomeric units used in the construction of oligodeoxyribonucleotides by the phosphite triester method. © 1986.

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Toren, P. C., Betsch, D. F., Weith, H. L., & Coull, J. M. (1986). Determination of impurities in nucleoside 3′-phosphoramidites by fast atom bombardment mass spectrometry. Analytical Biochemistry, 152(2), 291–294. https://doi.org/10.1016/0003-2697(86)90411-2

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