Determination of methylamphetamine and related compounds in human urine by high performance liquid chromatography/electrospray/mass spectrometry

21Citations
Citations of this article
4Readers
Mendeley users who have this article in their library.
Get full text

Abstract

A study of liquid chromatography/electrospray/mass spectrometry (LC/ES/MS) for the determination of methylamphetamine and related compounds (amphetamine, ephedrine, phenylpropanolamine) in human urine was undertaken. We assessed the effect of collision induced dissociation (CID) spectra generated by varying exit voltage of capillary and skimmer. The responses of ES/MS in different mobile phase and the effects of mobile phase modifier were examined. An isocratic LC method using methanol/water (80/20) and acetic acid (0.001%) as a modifier to separate these compounds was developed. Microporous ultrafiltration technique was employed to pre-treat urine sample prior to LC/ES/MS analysis. Good recoveries for methylamphetamine and amphetamine were determined as well as linearity, detection limit and precision associated with this method were determined. Drug spiked urine samples and urine samples of methylamphetamine addicts were successfully measured by this newly developed method.

Cite

CITATION STYLE

APA

Fuh, M. R. S., & Lu, K. T. (1999). Determination of methylamphetamine and related compounds in human urine by high performance liquid chromatography/electrospray/mass spectrometry. Talanta, 48(2), 415–423. https://doi.org/10.1016/S0039-9140(98)00264-1

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free