Determination of tryptamine in rat brain by gas chromatography-mass spectrometry

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Abstract

Tryptamine (TA) occurs in trace levels in the brain, but its role in the central nervous system is not clear. However, there is evidence that TA may be a neuromodulator since it binds to specific binding sites in the brain. TA was measured as a diheptafluorobutyryl derivative in rat whole brain by capillary gas chromatography-mass spectrometry using negative chemical ionization (NCI) and single ion monitoring (SIM). d4-TA was used as the internal standard. The ions m/z 532 and m/z 536 were monitored to identify TA and d4-TA, respectively and to calculate the concentration of TA in rat whole brain which was found to be 0.19 ± 0.08 ng g-1(n = 8). The results confirm the earlier TA concentrations measured by GC-MS using positive electron impact ionization. However, NCI improved the signal/noise ratio of the method increasing its sensitivity for TA. © 1988.

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Peura, P., Faull, K. F., & Barchas, J. D. (1988). Determination of tryptamine in rat brain by gas chromatography-mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 6(6–8), 821–825. https://doi.org/10.1016/0731-7085(88)80097-9

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