Determination of xylose oligomers and monosaccharides by anion-exchange chromatography with pulsed amperometric detection

  • Shifen M
  • Qun S
  • Depei L
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Anion-exchange chromatography followed by pulsed amperometric detection (AE-PAD) was applied to the determination of xylose oligomers and some monosaccharides. Under alkaline conditions, underivatized xylose oligomers (DP1-DP7; DP = degree of polymerization) were readily separated within 12 min. Underivatized monosaccharides (fucose, galactose, glucose, mannose, arabinose, xylose, galactosamine and glucosamine) were separated within 30 min at an eluent flow-rate of 1 ml min-1. The limits of determination of xylose oligomers were from 0.08 to 0.01 nmol. Results for both xylose oligomers and monosaccharide determination by AE-PAD were more sensitive than those obtained by conventional high-performance liquid chromatography. This method was also employed to determine the monosaccharides in pretreated peat samples. © 1991.

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  • Mou Shifen

  • Sun Qun

  • Lu Depei

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