Deuterium nuclear magnetic resonance study of the interaction of branched chain compounds (phytanic acid, phytol) with a phospholipid model membrane

  • Yue J
  • Thewalt J
  • Cushley R
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Abstract

Deuterium nuclear magnetic resonance (2H-NMR) spectra have been determined for 50 wt% aqueous dispersions of 1-palmitoyl(stearoyl)-2-[2H31]palmitoyl-sn-glycero-3-phosphocholine (PC-d31) containing 20 mol% of the isoprenoid compounds phytol or phytanic acid over the temperature range -5-55°C. Concentration effects of the isoprenoid compounds are also reported. First moments (M1) and order parameters were calculated from the spectra. 20 Mol% of either branched chain compound causes an approximate 9% increase in the mean order parameterCD. Significant effects are seen on the PC-d31phase behavior. 20 Mol% of either branched chain compound causes the gel to liquid crystalline onset temperature (Ts) to drop to 28°C from 38°C for PC-d31alone, as seen from the temperature dependent M1values. The melting range (|Ti-Ts|) is {reversed tilde equals}1.5°C for PC-d31and {reversed tilde equals}11°C for PC-d31containing 20 mol% of the branched chain compounds. This is in direct contrast to their straight chain analogues, hexadecanol and palmitic acid, which have been shown to elevate the phase transition temperature. The isoprenoid compounds cause significant disruption of the gel phase, forcing nearest neighbor phospholipid chains apart. Transverse relaxation times (T2e, the time constant for decay of the quadrupolar echo) have been determined over the temperature range -5-50°C. Possible explanation for the effect of the isoprenoid compounds on the dynamic structure of phospholipids in the bilayer are proffered. © 1988.

Author-supplied keywords

  • Deuterium NMR
  • differential scanning calorimetry
  • membranes
  • moment analysis
  • phosphatidylcholine
  • phytanic acid
  • phytol

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Authors

  • Junshi Yue

  • Jenifer L. Thewalt

  • Robert J. Cushley

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