Diacylglycerol regulates the plasma membrane calcium pump from human erythrocytes by direct interaction

  • Pérez-Gordones M
  • Lugo M
  • Winkler M
 et al. 
  • 4


    Mendeley users who have this article in their library.
  • 16


    Citations of this article.


The plasma membrane Ca2+-ATPase (PMCA) plays a key role in the regulation of the intracellular Ca2+concentration. Ethanol stimulates this Ca2+pump in an isoform-specific manner. On search for a physiological molecule that could mimic the effect of ethanol, we have previously demonstrated that some sphingolipids containing free "hydroxyl" groups, like ceramide, are able to stimulate the PMCA. Since diacylglycerol (DAG) structurally shares some characteristics with ceramide, we evaluate its effect on the PMCA. We demonstrated that DAG is a potent stimulator of this enzyme. The activation induced is additive to that produced by calmodulin, protein-kinase C and ethanol, which implies that DAG interacts with the PMCA through a different mechanism. Additionally, by different fluorescent approaches, we demonstrated a direct binding between PMCA and DAG. The results obtained in this work strongly suggest that DAG is a novel effector of the PMCA, acting by a direct interaction. © 2009.

Author-supplied keywords

  • Ca2+
  • Calmodulin
  • Diacylglycerol
  • PMCA
  • Protein-kinase C

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Gustavo BenaimFundacion Instituto de Estudios Avanzados

  • Maria C. Pérez-Gordones

  • Miguel R. Lugo

  • Moraima Winkler

  • Vincenza Cervino

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free