Different mechanisms of TEM-1 and Oxa-1 mediated resistance to piperacillin in E. coli

3Citations
Citations of this article
1Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Clinical isolates of Oxa-1 and TEM-1 producing strains of E. coli were studied. Susceptibility to piperacillin was determined by the agar and broth dilution procedure, and β-lactam hydrolysis rates measured by the iodometric method. The β-lactamases were identified by isoelectric focusing. Our data on TEM-1 producing strains showed a statistically significant correlation between the MIC, if determined by the agar dilution test, and the specific β-lactamase activity. The majority of Oxa-1 producing E. coli was resistant to piperacillin although the inactivation rate of piperacillin was usually low. Cell wall permeability of TEM-1 producing strains of E. coli to piperacillin was below the lower limit of detectability, but preincubation of the E. coli strains in piperacillin containing broth led to increased cell wall permeability. Bactericidal kinetics of an Oxa-1 and TEM-1 E. coli were studied. It revealed that regrowth of the Oxa-1 strain in piperacillin containing broth was associated with a 25 % decrease of piperacillin concentrations without the formation of degradation products, suggesting binding of piperacillin to bacterial cells. The TEM-1 plasmid bearing strain inactivated piperacillin, and the degradation products (penicilloate) could be detected. © 1984, Gustav Fischer Verlag, Stuttgart/New York. All rights reserved.

Cite

CITATION STYLE

APA

Marre, R. (1984). Different mechanisms of TEM-1 and Oxa-1 mediated resistance to piperacillin in E. coli. Zentralblatt Fur Bakteriologie Mikrobiologie Und Hygiene - Abt. 1 Orig. A, 258(2–3), 287–295. https://doi.org/10.1016/S0176-6724(84)80047-4

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free