When stimulated with immune complex or C5a anaphylatoxin, human neutrophils undergo an increase in the concentration of intracellular Ca2+([Ca2+]i) that precedes the onset of superoxide (O-2) production. The extracellular Ca2+is required for the O-2production of neutrophils stimulated by C5a, but is only partially required for those by immune complex. The addition of pertussis toxin to neutrophils does not inhibit the rise in [Ca2+]iand O-2production induced by immune complex but does inhibit those induced by C5a. These results suggest that a different sequence of reaction is involved by different stimulants, the anaphylatoxin and immune complex. © 1988.
Shirato, M., Takahashi, K., Nagasawa, S., & Koyama, J. (1988). Different sensitivities of the responses of human neutrophils stimulated with immune complex and C5a anaphylatoxin to pertussis toxin. FEBS Letters, 234(1), 231–234. https://doi.org/10.1016/0014-5793(88)81341-3