Differential effects of quercetin and resveratrol on Band 3 tyrosine phosphorylation signalling of red blood cells

  • Maccaglia A
  • Mallozzi C
  • Minetti M
  • 2

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Abstract

The protective effects of eating fruits and vegetables in the prevention of several degenerative pathologies have been attributed at least in part to the antioxidant and anti-inflammatory properties of polyphenols. In this study, we investigated the effects of two polyphenols, quercetin and resveratrol, on red blood cell Band 3 tyrosine phosphorylation signalling activated by peroxynitrite. Peroxynitrite is a physiological oxidant scavenged largely by the erythrocyte and formed by the reaction between nitrogen monoxide and superoxide anion. Quercetin and its structurally analogous (+)-catechin inhibited the peroxynitrite-dependent upregulation of Band 3 tyrosine phosphorylation. Quercetin was found to downregulate the activity of syk, which is upstream in the Band 3 tyrosine phosphorylation cascade, and partially prevented peroxynitrite-mediated phosphotyrosine phosphatase inhibition. Resveratrol and hydroxytyrosol, unexpectedly, amplified peroxynitrite-dependent upregulation of Band 3 tyrosine phosphorylation through the activation of lyn, a kinase of the src family. The present results clearly indicate that polyphenols may activate cell transduction pathways in different and sometimes opposite ways.

Author-supplied keywords

  • Anion Exchange Protein 1, Erythrocyte/chemistry/*m
  • Antioxidants/*pharmacology
  • Dose-Response Relationship, Drug
  • Drug Synergism
  • Enzyme Precursors/drug effects/metabolism
  • Erythrocyte Membrane/enzymology
  • Erythrocytes/drug effects/*metabolism
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Peroxynitrous Acid/antagonists & inhibitors/pharma
  • Phosphorylation
  • Protein Tyrosine Phosphatases/metabolism
  • Protein-Tyrosine Kinases/drug effects/metabolism
  • Quercetin/*pharmacology
  • Signal Transduction/drug effects
  • Stilbenes/*pharmacology
  • Tyrosine/*metabolism
  • src-Family Kinases/drug effects/metabolism

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Authors

  • A Maccaglia

  • C Mallozzi

  • M Minetti

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