SBA and NADPH-d histochemistries allow identification of functionally distinct components of the amphibian primary olfactory system. In Pipa, a secondarily aquatic frog, combination of both methodologies, using alternate sets of histological sections, reveals that, apart from Jacobson's organ, this species has a 'water-nose' and an 'air-nose'. The epithelia occupy separate chambers of the olfactory organ and give rise to olfactory nerve fiber bundles that are identified by the dual staining procedure.
Meyer, D. L., Fackler, I. R., Jadhao, A. G., D’Aniello, B., & Kicliter, E. (1997). Differential labelling of primary olfactory system subcomponents by SBA (lectin) and NADPH-d histochemistry in the frog Pipa. Brain Research, 762(1–2), 275–280. https://doi.org/10.1016/S0006-8993(97)00507-6