Discrimination between the regioisomeric 1,2- and 1,3- diacylglycerophosphocholines by phospholipases

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Abstract

The artificial 1,3-diacyl-glycero-2-phosphocholines (1,3-PCs), which form similar aggregate structures as the naturally occurring 1,2-diacyl-sn-glycero-3- phosphocholines (1,2-PCs), were tested as substrates for different classes of phospholipases such as phospholipase A2(PLA2) from porcine pancreas, bee and snake venom, and Arabidopsis thaliana, phospholipase C (PLC) from Bacillus cereus, and phospholipase D (PLD) from cabbage and Streptomyces species. The regioisomers of the natural phospholipids were shown to bind to all investigated phospholipases with an affinity similar to the corresponding naturally occurring phospholipids, however their hydrolysis was reduced to different degrees (PLA2s and PLC) or even abolished (PLDs belonging to the PLD superfamily). The results are in accordance with binding models obtained by docking the substrates to the crystal structures or homology models of the phospholipases. © 2011 Elsevier Ireland Ltd.

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Mansfeld, J., Brandt, W., Haftendorn, R., Schöps, R., & Ulbrich-Hofmann, R. (2011). Discrimination between the regioisomeric 1,2- and 1,3- diacylglycerophosphocholines by phospholipases. Chemistry and Physics of Lipids, 164(3), 196–204. https://doi.org/10.1016/j.chemphyslip.2010.12.009

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