There were significant levels of in vitro hydrogenase activity in Methanosarcina strains. The multiple forms of hydrogenase were observed in cell free extracts of cells grown on methanol. Strains having poor growth on H2: CO2had four forms while strains having normal growth on all substrates contained two forms of hydrogenase. These multiple forms differ in their charges as well as in their composition of transition metal ions. The strain having normal growth showed higher incorporation of63Ni2+and65Zn2+. Both hydrogenases, A and D, of strain P3had methylviologen and F420-reducing activity and contained Zn2+and Co2+respectively. Hydrogenases A and D of strains P1and P4also had similar characteristics whereas hydrogenases B and C had only methylviologen reducing activity. © 1990 Federation of European Microbiological Societies.
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