On the disulfide-linker strategy for designing efficacious cationic transfection lipids: An unexpected transfection profile

  • Vinod Kumar V
  • Chaudhuri A
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Herein, employing a previously reported disulfide-linker strategy, we have designed and synthesized a novel cationic lipid 2 with a disulfide-linker and its non-disulfide control analog lipid 1. The relative efficacies of lipids 1 and 2 in transfecting CHO, COS-1 and MCF-7 cells were measured using both reporter gene and whole cell histochemical staining assays. In stark contrast to the expectation based on the disulfide-linker strategy, the control non-disulfide cationic lipid 1 showed phenomenally superior in vitro transfection efficacies to its essentially transfection incompetent disulfide counterpart lipid 2. Results in DNase I protection experiments and the electrophoretic gel patterns in the presence of glutathione, taken together, are consistent with the notion that the success of the disulfide-linker strategy may depend more critically on the DNase I sensitivity of the lipoplexes than on the efficient DNA release induced by intracellular glutathione pool. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

Author-supplied keywords

  • DCC, dicyclohexyl carbodiimide
  • DCM, dichloromethane
  • DMEM, Dulbecco's modified Eagle's medium
  • DMF, dimethyl formamide
  • FBS, fetal bovine serum
  • HCl, hydrochloric acid
  • NHS, N-hydroxy succinimide
  • ONPG, o-nitrophenyl-β-D- galactopyranoside

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  • Valluripalli Vinod Kumar

  • Arabinda Chaudhuri

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