Herein, employing a previously reported disulfide-linker strategy, we have designed and synthesized a novel cationic lipid 2 with a disulfide-linker and its non-disulfide control analog lipid 1. The relative efficacies of lipids 1 and 2 in transfecting CHO, COS-1 and MCF-7 cells were measured using both reporter gene and whole cell histochemical staining assays. In stark contrast to the expectation based on the disulfide-linker strategy, the control non-disulfide cationic lipid 1 showed phenomenally superior in vitro transfection efficacies to its essentially transfection incompetent disulfide counterpart lipid 2. Results in DNase I protection experiments and the electrophoretic gel patterns in the presence of glutathione, taken together, are consistent with the notion that the success of the disulfide-linker strategy may depend more critically on the DNase I sensitivity of the lipoplexes than on the efficient DNA release induced by intracellular glutathione pool. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Vinod Kumar, V., & Chaudhuri, A. (2004). On the disulfide-linker strategy for designing efficacious cationic transfection lipids: An unexpected transfection profile. FEBS Letters, 571(1–3), 205–211. https://doi.org/10.1016/j.febslet.2004.06.058