Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line

  • Caruso M
  • Messa C
  • Orlando A
 et al. 
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Abstract

The phytoestrogen genistein has been demonstrated to possess anti-tumor properties by mechanisms not yet clearly established. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 μM to 50 μM on the LDL receptor and HMGCoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMGCoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an early increase of LDL receptor gene expression and later decreased HMGCoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMGCoA reductase gene expression in colon cancer. © 2008 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Colon cancer
  • Genistein
  • HMGCoA reductase
  • LDL receptor

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Authors

  • Maria Gabriella Caruso

  • Caterina Messa

  • Antonella Orlando

  • Benedetta D'Attoma

  • Maria Notarnicola

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