Effect of aflatoxin B1on in vitro production of interleukin-1 by bovine mononuclear phagocytes

  • Kurtz R
  • Czuprynski C
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Abstract

In this study we examined the effects of preincubation with aflatoxin B1(AFB1) on the ability of bovine monocytes to produce the immunological mediator interleukin-1 (IL-1). Monocytes preincubated for 6-24 h with 10 μg ml-1AFB1demonstrated a diminished capacity to release IL-1 activity in response to bacterial lipopolysaccharide (LPS). Preincubation for less time, or with lower concentrations of AFB1, did not affect IL-1 release. Pretreatment of monocytes with AFB1also resulted in diminished release of IL-1 activity in response to in vitro infection with Listeria monocytogenes. Incubation with AFB1reduced the amount of IL-1 β mRNA in LPS-stimulated bovine monocytes; however, this was observed only at high concentrations of AFB1that non-specifically reduced steady-state transcription of actin mRNA. We therefore concluded that AFB1does not specifically suppress monocyte release of IL-1, other than through its general inhibition of mRNA transcription. © 1992.

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Authors

  • Robin S. Kurtz

  • Charles J. Czuprynski

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