Effect of elicitation on the accumulation of solasodine by immobilized cells of Solanum eleagnifolium Cav.

  • Quadri L
  • Giulietti A
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Abstract

The effect of a fungal elicitor obtained from Alternaria sp. on growth and solasodine production by free and alginate-entrapped cells of Solanum eleagnifolium Cav. was studied. Fourteen-day-old cultures were elicited with 1% FW/V autoclaved homogenates. The solasodine production increased from 0.9 to 1.5 mg g-1DW (65%) in suspension cultures and from 0.75 to 1.4 mg g-1DW (about 95%) in entrapped cells. The maximum accumulation was obtained after 72 h of elicitation. In order to induce alkaloid release from cells (suspension and entrapped cells), permeabilization with 10% dimethylsulfoxide (DMSO) for 30 min was used. In both cases (free and entrapped cells), about 50-60% of intracellular solasodine was released into the medium. The reuse of elicited and permeabilized entrapped cells was also carried out for three production cycles. © 1993.

Author-supplied keywords

  • Solanum eleagnifolium Cav.
  • elicitation
  • immobilized cells
  • solasodine production

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Authors

  • L. E.N. Quadri

  • A. M. Giulietti

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