Effect of enteral diet supplemented with arginine on anti-inflammatory cytokines after thermal injury in rats

2Citations
Citations of this article
4Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Male Wistar rats weighing about 200g underwent catheter jejunostomy and received scald bums covering 30% of the whole-body surface area. Animals were divided into two groups: a control group (no supplemental arginine, n = 8) and an arginine group (supplemental arginine: 7.7 g/L, n = 8), both of whom received continuously total enteral nutrition for 7 days (250 kcal/kg/day, 1.72 gN/kg/day). The mRNA expression of interleukin-2 (IL-2), IL-10, and transforming growth factor-betal (TGF-β1) in the spleen, thymus, lungs, and liver was measured by a semiquantitative reverse transcription-polymerase chain reaction method. The concentration of the cytokines in plasma and supernatant of the cultured splenic lymphocytes was determined by enzyme-linked immunosorbent assay. The enteral diet supplemented with arginine significantly decreased the messenger RNA expression for IL-2 and TGF-β1 in the spleen (p < 0.05, p < 0.01), and for IL-2 in the lungs (p < 0.05) in burned rats when compared with the control diet. Moreover, the level of IL-2 in the supernatant from the cultured splenic lymphocytes was significantly suppressed in animals fed the arginine-supplemented diet (p < 0.05). These results suggest that dietary arginine supplementation decreases the mRNA expression and production of anti-inflammatory cytokines after thermal injury. © 2001 Elsevier Science rfInc. All rights reserved.

Cite

CITATION STYLE

APA

Cui, X. L., Iwasa, M., Morisawa, K., Sasaguri, S., & Ogoshi, S. (2001). Effect of enteral diet supplemented with arginine on anti-inflammatory cytokines after thermal injury in rats. Nutrition Research, 21(8), 1171–1183. https://doi.org/10.1016/S0271-5317(01)00318-9

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free