The effects of lysophosphatidic acid (LPA) on ovum transport in mouse oviducts were studied. When excised oviducts were incubated at 37°C under 5% CO2in humidified air for 24 hours, addition of LPA at 10 μM to the medium significantly accelerated the rate of ovum transport, and 1 μM LPA slightly increased the ovum transport rate. These increases were not inhibited by 10 μM indomethacin, a cyclooxygense inhibitor, but were suppressed by 200 ng/ml of pertussis toxin or 10 μM verapamil, a voltage-sensitive calcium channel blocker. These data suggested that LPA stimulates mouse ovum transport by contracting oviductual smooth muscle via a voltage-sensitive calcium channel mediated by a pertussis toxin-sensitive G-protein-linked receptor.
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