1. 1. Decreased staining intensity with the Alcian Blue-Alcian Yellow technique for neurosecretory material led to a reduction in the visible numbers of Yellow Cells and Yellow-green Cells in brains from snails exposed to deionized water for more than 2 days. compared with snails from standard tapwater. 2. 2. A significant drop in blood Na+and Cl-concentrations preceded the histological change in Yellow Cell and Yellow-green Cell appearance. 3. 3. A significant drop in blood volume only occurred after 4 days exposure to deionized water, rather delayed with respect to the above histological and physiological changes. © 1978.
Soffe, S. R., Benjamin, P. R., & Slade, C. T. (1978). Effects of environmental osmolarity on blood composition and light microscope appearance of neurosecretory neurones in the snail, Lymnaea stagnalis (L.). Comparative Biochemistry and Physiology -- Part A: Physiology, 61(4), 577–584. https://doi.org/10.1016/0300-9629(78)90131-7