Effects of poly(l-lysine) on the structural and thermotropic properties of dipalmitoylphosphatidylglycerol bilayers

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Abstract

The effects of poly(l-lysine) on the structural and thermotropic properties of dipalmitoylphosphatidylglycerol (DPPG) bilayers were studied with differential scanning calorimetry (DSC), X-ray diffraction and freeze-fracture electron microscopy. For thermal behavior, in the DPPG/poly(l-lysine) system the main transition temperature rises to 45.7°C and the pretransition disappears in opposition to pure DPPG vesicles. An additional transition appears approximately at 36°C for the DPPG/poly(l-lysine) system after incubation at 4°C for two months. The incubated sample gives a X-ray diffraction pattern having several additional reflections in the range of 0.2-0.9 nm at 15°C. These results suggest that even in the presence of poly(l-lysine) the DPPG bilayers form the subgel (Lc) phase after the long incubation at a low temperature. The X-ray diffraction measurements indicate that the structure of the Lc phase for DPPG/poly(l-lysine) system is different from that of pure DPPG bilayers. On the other hand, in the gel (Lβ′) phase, the wide-angle X-ray diffraction pattern suggests that the presence of poly(l-lysine) hardly affects the packing of hydrocarbon chains in the DPPG bilayers. The small-angle X-ray diffraction and freeze-fracture electron microscopy exhibit that the DPPG/poly(l-lysine) system forms a tightly packed multilamellar structure in which the poly(l-lysine) is intercalated between the subsequent DPPG bilayers. © 1992.

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Takahashi, H., Matuoka, S., Kato, S., Ohki, K., & Hatta, I. (1992). Effects of poly(l-lysine) on the structural and thermotropic properties of dipalmitoylphosphatidylglycerol bilayers. BBA - Biomembranes, 1110(1), 29–36. https://doi.org/10.1016/0005-2736(92)90290-3

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