Electron transport reactions in a cytochrome c-deficient mutant of Paracoccus denitrificans

  • Bolgiano B
  • Smith L
  • Davies H
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A mutant of Paracoccus denitrificans which is deficient in c-type cytochromes grows aerobically with generation times similar to those obtained with a wild-type strain. The aa3-type oxidase is functional in the mutant as judged by spectrophotometric assays of cytochrome c oxidation using the membrane particles and cytochrome aa3reduction in whole cells. The cytochrome c oxidase (aa3-type) of the c-less mutant oxidizes soluble cytochrome c at rates equivalent to those obtained with the wild-type. NADH and succinate oxidase activities of the membrane preparations of the mutant and wild-type are also comparable in the absence of detergent treatment. Exogenous soluble cytochrome c can be both reduced by NADH- and succinate-linked systems and oxidized by cytochrome aa3present in membranes of the mutant strain. Rapid overall electron transport can occur in the c-less mutant, suggesting that reactions result from collision of diffusing complexes. © 1989 Elsevier Science Publishers B.V. (Biomedical Division) All rights reserved.

Author-supplied keywords

  • (P. denitrificans)
  • Cytochrome c
  • Cytochrome-c oxidase
  • Cytochrome-c reductase
  • Electron transport
  • Respiratory chain

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  • Barbara Bolgiano

  • Lucile Smith

  • Helen C. Davies

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