Electrophoretic identification of fusion proteins expressed in single recombinant λ-bacteriophage plaques

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Abstract

Sufficient fusion protein is present in single plaques produced by lytic, recombinant λ bacteriophage to be detected by Coomassie blue staining following electrophoresis on sodium dodecyl sulfate-polyacrylamide gels. Agar plugs containing single plaques can be loaded directly onto the stacking gel thus avoiding the need for extensive sample preparation. © 1986.

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Saul, A., & Yeganeh, F. (1986). Electrophoretic identification of fusion proteins expressed in single recombinant λ-bacteriophage plaques. Analytical Biochemistry, 156(2), 354–356. https://doi.org/10.1016/0003-2697(86)90265-4

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