Electrophoretic identification of fusion proteins expressed in single recombinant λ-bacteriophage plaques

  • Saul A
  • Yeganeh F
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Abstract

Sufficient fusion protein is present in single plaques produced by lytic, recombinant λ bacteriophage to be detected by Coomassie blue staining following electrophoresis on sodium dodecyl sulfate-polyacrylamide gels. Agar plugs containing single plaques can be loaded directly onto the stacking gel thus avoiding the need for extensive sample preparation. © 1986.

Author-supplied keywords

  • bacterial culture
  • gel electrophoresis
  • gene expression
  • proteins
  • recombinant technology

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Authors

  • Allan Saul

  • Frederick Yeganeh

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