The entry of sugar beet cells into the G0state involves extensive re- programming of gene expression mechanisms via transcriptional and translational controls

  • Fowler M
  • Kirby M
  • Slater A
 et al. 
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Abstract

Sugar beet cells grown in liquid suspension culture entered the stationary phase on day 17 of the batch culture cycle as a consequence of nutrient depletion. Between days 17 and 19 the cells exhibited a number of marked changes in RNA and protein metabolism characteristic of entry into a quiescent or G0state. Protein synthesis declined considerably and very few polypeptides incorporated sufficient radioactively labelled methionine to be detected by fluorography following polyacrylamide gel electrophoresis (SDS- PAGE). There was a marked decrease in protein half life during the stationary phase and this was accompanied by a rise in protease activity. RNA synthesis also decreased to

Author-supplied keywords

  • Beta vulgaris
  • Cell division cycle
  • Differential display
  • G0phase
  • Protease
  • Protein synthesis
  • Protein turnover
  • Quiescence
  • RNA synthesis
  • Sugar beet

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